本研究選用子宮頸癌細胞株(Hela cell line)與正常細飽，探討兩者對奈米金的攝取量及對輻射敏感度之差異。在實驗上，將奈米金粒子(15 ppm)分別加入正常之骨髓細胞、周邊血液單核球以及Hela cell中共同培養，隔天測量細胞對奈米金粒子的攝取量，再以γ射線（0或5 Gy）照射細胞，並觀察細胞形態，比較細胞的凋亡率以及BMP基因之表現，進而探討奈米金粒子對骨髓細胞的分化是否產生影響。結果顯示，骨髓細胞的奈米金粒子攝取量爲Hela cell的50%，而周邊血液單核球則爲比Hela cell的59%。攝取奈米金的細胞，經輻射照射後，其凋亡率相較於未攝取者，各爲：Hela cell(183%)、骨髓細胞(172%)、周邊血液單核球(154%)。僅攝取奈米金，但未照射輻射的細胞，則兩者凋亡率沒有統計學上的差異。此外在骨髓細胞分化的實驗中，發現奈米金可增加含鹼性磷酸酶的細胞比率，且提高骨頭的生成量。在基因表現分析中，發現奈米金粒子可促進BMP基因的表現，若已攝取奈米金粒子的骨髓細胞經輻射照射，上述因子則會被抑制。由研究結果可知，奈米金粒子可提高Hela cell、骨髓細胞及週邊血液單核球細胞對輻射的敏感性，促進其凋亡。Hela cell對奈米金粒子的攝取量明顯較正常細胞多，經輻射照射後所引起的傷害也較正常細胞大。 Nano gold particles (≦20 nm in diameter) were cocultured with Hela cells and normal cells to investigate the difference between both cells in the intaken quantity toward nano gold particles and the sensitivity toward radiation. Experimentally, the nano gold particles (15 ppm) were respectively added into the culture media of bone marrow cells, peripheral blood monocytes and Hela cells. On the next day, the intaken quantity was measured. After irradiated with γ-ray (0 or 5 Gy), cell morphology observation as well as the comparison about cell apoptosis and BMP gene expression were carried out to investigate if nano gold particles have effect on the differentiation of bone marrow cells. The results indicated that the intaken quantity toward nano gold particles for bone marrow cells and peripheral blood monocytes was 50% and 59% respectively than that for Hela cells. When compared with cells without nano gold particle intake, the apoptosis rate for the irradiated cells intaking nano gold particles were 183%, 172% and 154% for Hela cells, bone marrow cells and peripheral blood monocytes respectively. No statistical difference in apoptosis rate was observed among the three kinds of cells. Besides, the nano gold particles were discovered able to increase the ratio of cells excreting alkaline phosphatase and to promote bone formation in the experiment of bone marrow cell differentiation. The nano gold particles were also observed able to enhance BMP gene expression in the analysis of gene expression. Provided that the bone marrow cells intaking nano gold particles were irradiated, the above factor was suppressed. According to the results, the nano gold particles were capable of potentiating radiation sensitivity of Hela cells, bone marrow cells and peripheral blood monocytes as well as to enhancing their apoptosis. The intaken quantity toward nano gold particles for Hela cells was apparently higher than normal cells, for which the radiation damage was less severe than that of Hela cell after intaking the particles.
關聯:
台灣應用輻射與同位素雜誌/Taiwanese Journal of Applied Radiation and Isotopes 4卷2期 pp.469-479
