本研究以低能量氦氖雷射照射骨髓細胞，觀察其對骨細胞分化及相關基因（破骨細胞分化因子及骨形態蛋白）之表現，以探討低能量氦氖雷射對小鼠骨量生成之影響。實驗中取小鼠脛骨與股骨之骨髓細胞，以低能量氦氖雷射（波長632nm）照射（0、10、15及20秒），培養3天後，收集培養液（作爲培養破骨細胞的condition medium），並分析骨形態蛋白及破骨細胞分化因子的基因表現。培養7天後，計算造骨細胞分化之百分比，於第14天統計骨小結(bono nodule)的生成數目，並於第30天觀察骨組織生成量。此外，以上述收集之condition medium培養骨髓細胞，並於培養後第14天統計破骨細胞的數量。結果顯示接受雷射照射組，其骨量較對照組多，且在細胞分化方面，破骨細胞的數目較少，但造骨細胞數目則較多。此外，低能量氦氖雷射可抑制ODF及促進BMP之基因表現。以此推測，低能量氦氖雷射可透過減少破骨細胞的分化及增加造骨細胞的分化，以增加骨量生成。 Bone marrow cells were stimulated with low-energy He/Ne laser in this study to investigate the effect on bone growth of mice from differentiation of bone cells and expressions of related genes of receptor activator of osteoclast differentiation factor (ODF) and bone morphogenic proteins-1 (BMP-1). In the experiment, bone marrow cells taken from tibia and femur bones were irradiated with low-energy He/Ne laser (wavelength=632 um) for 0, 10, 15 and 20 s and then cultured for 3 days. The culture medium was collected for osteoclast culture as a condition medium, and the gene expression of BMP and ODF was analyzed. After 7 days of culture, percentage of differentiation of osteoblasts was counted. Bone nodules formed were totaled on the 14(superscript th) day, and the mass of bone tissue was estimated. Bone marrow cells were cultured with the collected condition medium, and the amount of osteoclasts were counted on the 14(superscript th) day after culture. The results indicated that bone mass of the experimental group was higher than that of the control. Moreover, the number of osteoclasts was higher in experimental group than in the control; however, the number of osteoblasts exhibited reverse result. Additionally, gene expression of ODF was inhibited while that of BMP-1 was enhanced by low-energy He/Ne laser irradiation. Accordingly, low-energy He/Ne laser was suggested capable of increasing bone mass via suppressing differentiation of osteoclasts and increasing that of osteoblasts.
關聯:
台灣應用輻射與同位素雜誌/Taiwanese Journal of Applied Radiation and Isotopes 5卷2期 pp.669-675
